Genes for the protein antigens of the tuberculosis and leprosy bacilli.

نویسندگان

  • B R Bloom
  • V Mehra
  • R A Young
چکیده

The lambda gt 11 expression vector permitted us to survey protein antigens of Mycobacterium leprae and Mycobacterium tuberculosis expressed in Escherichia coli. Using monoclonal antibodies, recombinant clones were detected producing three major antigens of M. tuberculosis and five major protein antigens of M. leprae. These recombinant antigens produced in E. coli should prove useful for diagnosis, epidemiology and possibly the development of recombinant mycobacterial vaccines.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

THE DETECTION OF Mycobacterium tuberculosis BY PCR IN SPUTUM SAMPLES FROM LONG-TERMTREATED LEPROSY PATIENTS: ASSOCIATIONS WITH SKIN TEST RESULTS AND IMMUNOTHERAPY WITH Mycobacterium vaccae

As part of a series of investigations at Baba Baghi Leprosarium in lran, 44 long-treated leprosy patients were selected for our study. Samples of early morning sputum were obtained from each patient, examined by microscopy for acid-fast bacilli (AFB), and cultured for tubercle bacilli. These tests were negative, but the polymerase chain reaction (PCR) for an insertion sequence believed to b...

متن کامل

Leprosy and tuberculosis: a comparative study with the aid of skin tests with tuberculin, killed BCG, and the Dharmendra lepromin in South African Bantus.

The aim of this study was to investigate the immunological relationship between leprosy and tuberculosis, with a view to ascertaining the possible value of BCG vaccination in leprosy prophylaxis. The problem of the use of BCG for this purpose is dealt with in so many publications that we find it unnecessary to discuss the subject again, mentioning only certain important pUblications: by Fernand...

متن کامل

معرفی یک مورد جذام حنجره

    Introduction: Leprosy is an ancient deforming disease caused by Mycobacterium leprae, which is still poorly understood and often feared by the general public and even by some in the health care professions. Fortunately, the outlook for patients has dramatically improved over the last three decades with the introduction of multi-drug treatment and management strategies that have somewhat dim...

متن کامل

Genes for the major protein antigens of Mycobacterium tuberculosis: the etiologic agents of tuberculosis and leprosy share an immunodominant antigen.

Mycobacterium tuberculosis genes encoding immunologically relevant proteins were isolated by systematically screening a lambda gt11 recombinant DNA expression library with a collection of murine monoclonal antibodies directed against protein antigens of this pathogen. These antibodies, previously characterized by a World Health Organization workshop on monoclonal antibodies against mycobacteria...

متن کامل

Antibodies to mannophosphoinositides in leprosy patients.

Evidence has accumulated in recent years for the presence of common antigens in Mljcobacterium tuberculosis and Mljcohacteriunt leprae. Thus, Pepys et al. (9) , using precipitin tests found thrcc antigens common to the various PPD preparations and lepromin . Tuma and Silva ( IU) observed positive precipitins when antigens isolated from M. tuberculosis and BCG reacted with sera from rabbits immu...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Bioscience reports

دوره 5 10-11  شماره 

صفحات  -

تاریخ انتشار 1985